Figure 6.

C19orf12 expression in human cells. Representative Western blot analysis of C19orf12 expression in cellular lysates. Recombinant C19orf12 proteins (77, 141, 152 aa) were used as molecular weight references besides the protein ladder. Actin was used as a loading control. An amount of 10 μg of cellular lysate proteins and 1ng of recombinant proteins were loaded (A). Relative quantification of C19orf12 (141 aa) expression to actin. Chemiluminescent protein signals were quantified under no-saturating conditions (three seconds exposure time) using ImageJ (B). n = 4 independent experiments were considered for the quantification.