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. 2022 Dec 22;13(1):31. doi: 10.3390/life13010031

Table 1.

Primer sequences for site-directed mutagenesis.

Mutant Primer Sequences Amino Acid Change:
wt → Mutant
R248W Rev: GTTCATGCCGCCCATGCAGGAACTGTAACACATGTAGTTGTAGTGGATGGT
For: CCTGCATGGGCGGCATGAACtGGAGGCCCATCCTCACCATCATC		 CGG (R) → TGG (W)
R248Q Rev: GTTCATGCCGCCCATGCAGGAACTGTAACACATGTAGTTGTAGTGGATGGT
For: CTGCATGGGCGGCATGAACCaGAGGCCCATCCTCACCATCATC		 CGG (R) → CAG (Q)
R273H Rev: GCACCTCAAAGCTGTCCCGTCCCAGTAGATTACCACTGGAGTCTTCC
For: ACGGGACAGCTTTGAGGTGCaTGTTTGTGCCTGTCCTGGGAGA		 CGT (R) → CAT (H)