Table 2.

Melting temperatures of wild-type p53 and its mutant variants measured by differential scanning fluorimetry.

Protein	Structural Region	Tm, °C	ΔTm, °C *
p53 (wild-type)	-	42.9 ± 0.0	-
p53(Y220C)	β-sandwich	40.3 ± 0.2	−2.6
p53(R248Q)	DNA contact	38.5 ± 0.3	−4.4
p53(R248W)	DNA contact	39.3 ± 0.0	−3.6
p53(R273H)	DNA contact	38.8 ± 0.3	−4.1

* ΔTm = Tm (mutant) − Tm (wild-type). Mean values of triplicate measurements ± SEM are shown.