Skip to main content
. 2023 Jan 8;24(2):1246. doi: 10.3390/ijms24021246

Figure 1.

Figure 1

Media selection impacts epithelial and mesenchymal marker expression in wtCFTR and F508del CFBE41o- cells. RT qPCR was performed for e-cadherin, vimentin, zo-1, and n-cadherin in wtCFTR- (A) and F508del (B)-expressing CFBE41o- cells following growth in different media. Expression was normalized to actin and expressed as fold change relative to MEM media. Similar relative trends were noted for immunoblot of e-cadherin and vimentin in wtCFTR- (C) and F508del (D)-expressing cells. Statistical comparisons were made within the protein of interest (e.g., vimentin:vimentin), comparing each media to MEM. OD was normalized to C4 Actin. Light grey—E-Cadherin; dark grey—vimentin. Using these immunoblot data, an e-cadherin to vimentin ratio was calculated for wtCFTR- (E) and F508del (F)-expressing cells. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. MEM by one-way ANOVA with Dunnett’s multiple comparison test.