Figure 4.

Media selection impacts wtCFTR but not F508del CFTR function in CFBE41o- cells. Cells grown in four media were mounted in Ussing chambers and CFTR function was quantified under voltage clamp conditions. In wtCFTR+ cells, significantly higher CFTR function was present in cells grown in MEM (representative Ussing tracings in (A); aggregate data in (B)), while this differential function was not noted in F508del CFTR+ cells (representative Ussing tracings in (C); aggregate data in (D)). White bars—ΔcAMP; light grey—ΔGenistein; dark grey—ΔInh172. n = 3–4 inserts per condition. **** p < 0.0001 vs. MEM by one-way ANOVA with Dunnett’s multiple comparisons correction.