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Overwiew of attributes tested, methods applied and expected data ranges.
| Attribute | Method | Expected Result |
|---|---|---|
| pH | pH-metry | 6.5 ± 0.5 (Histidine buffer) |
| AlumOH aggregation profile |
Static Light Scattering | 1–20 μm |
| Antigen integrity and adsorption |
SDS-PAGE | No degradation and almost complete adsorption |
| AlumOH surface charge |
Zeta Potential | ≤0 (mV) |
| Antigen distribution | Flow Cytometry | Variation of Mean Fluorescence Intensity (MFI) |
