Figure 6.

Phenotype of EAV infected live MoDC. Immature MoDC (iMoDC) and mature MoDC (mMoDC) were inoculated at MOI of 5 with different strains of live or UV inactivated EAV. Controls were uninfected (media only) and mock infected cells. Cultures were incubated at 37 °C in 5% CO₂ for 16 h after which cells were harvested and stained to assess phenotype. Cells were stained with the live/dead fixable vioblue dead cell kit, followed by staining with anti-CD206-PE, anti-CD83-PECy5, anti-CD86-PECy5 and anti-MHC II-APC mAbs. Stained cells of the different treatments were assessed by comparing the same number of events (10,000) in the live cell target gate. (a) There were no biologically significant changes in the phenotype of EAV infected iMoDC. (b) The Velogenic strain significantly increased the expression of CD206 but decreased the expression of CD83 on mMoDC, thus rendering a more immature phenotype. There were no significant changes in the expression of CD86 and MHC II. Results were represented as the % surface marker expression of nine independent repeats ± SEM. A 2-way ANOVA with Bonferroni post-test was used to compare replicate means. *, ** and *** indicate significant differences between sample means where p < 0.05, p < 0.01 and p < 0.001, respectively.