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. 2023 Jan 16;15(1):255. doi: 10.3390/v15010255

Figure 7.

Figure 7

The expression level of costimulatory molecules on EAV infected Mo and MoDC. The co-stimulatory molecules, PD-L1/CD274, PD-L2/CD273, ICOS-L/CD275 and B7-H3/CD276, were assessed from EAV infected Mo and MoDC 16 hpi using duplex TaqMan qPCR assays with 18S rRNA gene as the reference. The normalized target gene expression was calculated by using the 2-ΔΔCt formula using the mock infected cells as comparator. There were significant differences in the expression of PD-L1/CD274 and PD-L2/CD273 on infected mMoDC with only the Attenuated and UK1 strains resulting in an upregulation (a,b). The overall expression differences of ICOS-L/CD275 on infected Mo and MoDC were low (less than 2-fold differences) (c). The expression level of B7-H3/CD276 on infected iMoDC and mMoDC was significantly different between strains (d). Results were represented as the average fold difference ± SEM (n = 3). A 2-way ANOVA with Bonferroni post-test was used to compare replicate means. *, ** and *** indicate significant differences between sample means where p < 0.05, 0.01 and 0.001, respectively.