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. 2023 Jan 9;13(1):197. doi: 10.3390/life13010197

Figure 4.

Figure 4

Methods for isolating CSC-like cells. A variety of different methods have been developed for isolating and enriching CSCs from different samples. Often these methods are combined to achieve efficient isolation of CSCs. (A) Selection based on the expression of certain cell-surface markers. (B) Isolation based on the ability of cells to efflux certain stains. (C) Enrichment based on the formation of 3D tumorspheres. (D) Isolation through determining ALDH enzymatic activity.