Table 1.
SSR markers sequences for chromosome arm location of 3DS and 3DL in wheat were used in this study. F indicated forward primer; R indicated reverse primer.
| Marker | Primer Sequences (5′-3′) | Annealing Temperature (°C) |
Chromosomal Arm Location |
Amplification Size (bp) |
|---|---|---|---|---|
| Xcfd55 | F: CCAGTAGCCGGCCCTACTAT R: GCACGAGATACGGACAATCA |
57 | 3DS | 269 |
| Xcfd141 | F: CGTAAAGATCCGAGAGGGTG R: TCCGAGGTGCTACCTACCAG |
58 | 3DS | 152 |
| Xcfd201 | F: ACAAGACCACACCTCCAAGG R: CGGTTTGGGTTTTGTGATCT |
55 | 3DL | 206 |
| Xwmc552 | F: ACTAAGGAGTGTGAGGGCTGTG R: CTCTCGCGCTATAAAAGAAGGA |
58 | 3DL | 149 |