FIG. 2.
Comparative binding of P. aeruginosa PAO1 and its fliD and fliC mutants to the polyacrylamide derivative bearing the sialyl-Lex glycotope. PAO1-D, fliD mutant of PAO1; PAO1-D(375 Db), PAO1-D complemented with the complete fliD gene on a multicopy plasmid vector, pPZ375Db; PAO1-D(375), PAO1-D with the vector pPZ375; PAO1-C, fliC mutant of PAO1. Differences in binding between strain PAO1 and its mutants marked by one asterisk were not significant, whereas those marked by two asterisks were considered significant (P < 0.05) by Student's t test. The binding of PAO1-D(375Db) and that of PAO1-D(375) were also significantly different.