TABLE 3.

Binding of fluorescent glycoconjugates bearing Le^x, sialyl-Le^x, and 6-sulfosialyl-Le^x to P. aeruginosa strain PAO1 and to its fliD mutants

Glycoconjugate	Kd (nM ± SD)a for strain:				Comparison of Kd valuesb
	PAO1	PAO1-D	PAO1-D(375Db)	PAO1-D(375)	PAO1 vs PAO1-D	PAO1 vs PAO1-D(375Db)
LPF	39 ± 7	70 ± 8	46 ± 8	64 ± 5	P < 0.005	NS
Sialyl-LPF	31 ± 3	61 ± 6	40 ± 10	67 ± 7	P < 0.005	NS
6-Sulfosialyl-LPF	59 ± 7	72 ± 9	77 ± 8	78 ± 10	NS	NS

^aThe number of data points for Scatchard analysis ranged between 20 and 30. 

^bNS, not significant. 

^cLPF, Le^x-PAA-Flu.