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. 2023 Jan 4;9(1):75. doi: 10.3390/jof9010075

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of mycelial growth of wild-type (WT) and mutant strains in A. oligospora. (A) Colony morphology of fungal strains incubated for 5 days on PDA, TG, and TYGA plates at 28 °C. (B) Diameters of fungal colonies in (A). (C) Hyphae of the WT and mutant strains stained with calcofluor white (CFW) and 4′,6-diamidino-2-phenylindole (DAPI) after 7-day incubation on CMY. One hundred hyphal cells were randomly selected for the counts of nuclei. Bar: 10 μm. (D,E) Length of hyphal cells and count of nuclei in (C). Measurements represent the average of three independent experiments. The asterisk indicates a significant difference between the mutant and WT strains (p < 0.05).