Figure 6.

Effect of FGFR3 gene silencing on constitutive expression of RAS and phosphorylation of MAPK signaling mediators in cultured UTUC cells. (A) BFTC-909 cells were treated with FGFR3-targeted siRNA or scramble non-target control (NTC) for 48 h and subjected to Western blotting detection. GAPDH was used as loading internal control. (B) Densitometry analyses for protein expression levels of FGFR3 (B) and RAS (C) are shown as ratios to GAPDH, and phosphorylation levels of EKR1/2 (D) and JNK1/2 (E) are shown as ratios to respective total proteins. Density data are expressed as mean ± SEM (n = 3). * indicates a p < 0.05 between the indicated groups.