FIG. 2.
IFN-γ production by SCID C3H/HeJ mouse spleen cells induced by LeIF. (A) Spleen cells (2 × 105/100 μl) were stimulated with different concentrations of LeIF and incubated for 48 h. Supernatants were harvested and tested by ELISA for the presence of IFN-γ. (B) Spleen cells were incubated with LeIF (10 μg/ml) or LPS (1 μg/ml) with or without polymyxin B (10 μg/ml), and supernatants were harvested after 48 h and assayed for the presence of IFN-γ. Results represent the arithmetic mean of duplicate culture supernatants in one of five experiments with essentially the same results.