FIG. 8.
Selective Th1 cytokine production by spleen cells of SCID C3H/HeJ mice stimulated with LeIF. Spleen cells (2 × 105/100 μl) were stimulated with different concentrations of LeIF and incubated for 48 h. As a control, cells were stimulated with 2 ng of PMA per ml plus 2 ng of ionomycin per ml. Supernatants were harvested and tested by ELISA for the presence of IFN-γ and IL-5. Results represent the arithmetic mean of duplicate culture supernatants in one of two experiments with essentially the same results.