Table 2.
The list of proteins distinguishing IUI from IVF cohorts. Those proteins were quantified in at least two replicates of all samples and have Wilcoxon test p values below 0.05. The protein intensities in the IUI and IVF columns are reported as medians with the minimum and maximum values of the log2-transformed aggregated intensity shown in parentheses. Log2 fold changes were calculated based on the median values and are reported as IUI/IVF ratios. Tissue expression data are from the Human protein atlas [20].
| Protein | Uniprot ID | Gene Symbol | IUI | IVF | Log 2 Fold Change | Wilcox Test p-Value | Tissue Expression |
|---|---|---|---|---|---|---|---|
| WAP four-disulfide core domain protein 2 | Q14508 | WFDC2 | 22.23 (21.61–23.18) | 28.58 (28.07–30.04) | −6.35 | 0.00009 | Tissue enhanced (cervix, salivary gland) |
| Enoyl-CoA hydratase | H0YFD6 | HADHA | 27.4 (26.34–28.32) | 22.5 (21.77–24.36) | 4.9 | 0.00015 | Tissue enhanced (skeletal muscle) |
| Dopamine receptor interacting protein 4 | Q4W4Y1 | DRIP4 | 24.65 (23.99–29.22) | 21.67 (21.64–22.17) | 2.98 | 0.00026 | N/A |
| 26S proteasome non-ATPase regulatory subunit 1 | Q99460 | PSMD1 | 20.81 (20.02–21.45) | 23.23 (22.66–23.39) | −2.42 | 0.00097 | Low tissue specificity |
| Serpin family A member 3 | A0A024R6P0 | SERPINA3 | 23.81 (22.91–24.6) | 26.63 (25.33–26.82) | −2.82 | 0.00097 | Group enriched (liver, pancreas) |
| Connective tissue growth factor | Q5M8T4 | CTGF | 24.38 (22.83–25.49) | 20.38 (20.24–20.76) | 4 | 0.00145 | N/A |
| Glucose-6-phosphate isomerase | P06744 | GPI | 24.68 (23.75–31.52) | 22.16 (21.69–22.44) | 2.52 | 0.00145 | Low tissue specificity |
| Laminin subunit gamma-2 | Q13753 | LAMC2 | 25.02 (23.24–25.35) | 21.37 (21.04–21.54) | 3.65 | 0.00210 | Tissue enhanced (urinary bladder) |
| Serpin family A member 5 | P05154 | SERPINA5 | 23.34 (22.86–24.34) | 26.11 (24.62–26.71) | −2.77 | 0.00299 | Tissue enhanced (adrenal gland, liver, testis) |
| Phosphatidylinositol 4-kinase alpha | P42356 | PI4KA | 22.81 (21.58–23.46) | 20.85 (20.43–21.29) | 1.96 | 0.00299 | Low tissue specificity |
| Antileukoproteinase | P03973 | SLPI | 29.09 (28.37–30.85) | 31.87 (31.66–32.18) | −2.78 | 0.00567 | Group enriched (cervix, salivary gland) |
| Stratifin | P31947 | SFN | 22.25 (20.94–23) | 24.7 (23.61–25.3) | −2.45 | 0.00567 | Group enriched (esophagus, skin, vagina) |
| Polymeric immunoglobulin receptor | P01833 | PIGR | 26.09 (25.25–27.66) | 29.5 (28.89–29.86) | −3.41 | 0.00567 | Tissue enhanced (intestine, salivary gland) |
| Midkine | P21741 | MDK | 21.56 (21.19–23.06) | 24.15 (23.88–24.52) | −2.59 | 0.00762 | Tissue enhanced (ovary) |
| Complement factor D | Q6FHW3 | DF | 22.67 (21.52–23.63) | 27.12 (23.95–27.79) | −4.45 | 0.01013 | N/A |
| Heat shock-related 70 kDa protein 2 | P54652 | HSPA2 | 22.49 (21.58–23.44) | 20.81 (20.43–21.49) | 1.68 | 0.01013 | Tissue enhanced (brain, skeletal muscle) |
| Elongation factor 1-gamma | P26641 | EEF1G | 25.04 (23.62–25.65) | 21.2 (19.37–23.49) | 3.84 | 0.01013 | Low tissue specificity |
| FLJ00385 protein | Q8NF17 | FLJ00385 | 28.94 (26.87–29.81) | 26.31 (24.04–26.89) | 2.63 | 0.01013 | N/A |
| Cystatin C | A0A0K0K1J1 | CSTS3 | 24.16 (23.61–25.99) | 28.73 (27.02–28.83) | −4.57 | 0.01327 | Tissue enhanced (brain) |
| Ubiquitin-conjugating enzyme E2D 3 | A0A024RDH2 | UBE2D3 | 24.12 (23.36–25.01) | 21.1 (19.86–22.89) | 3.02 | 0.01327 | Low tissue specificity |
| Immunoglobulin delta heavy chain | P0DOX3 | N/A | 23.74 (22.24–27) | 21.19 (20.28–21.82) | 2.55 | 0.01327 | N/A |
| Lysine--tRNA ligase | Q15046 | KARS1 | 21.5 (20.19–22.1) | 23.81 (21.76–24.82) | −2.31 | 0.01721 | Low tissue specificity |
| Talin-1 | Q9Y490 | TLN1 | 22.05 (21.48–23.09) | 23.87 (22.72–24.8) | −1.82 | 0.02202 | Low tissue specificity |
| Myosin regulatory light chain 11 | Q96A32 | MYL11 | 22.61 (21–23.08) | 20.21 (19.59–21.23) | 2.4 | 0.02202 | Group enriched (skeletal muscle, tongue) |
| Dermcidin | P81605-2 | DCD | 24.36 (22.79–27.5) | 22.47 (21.57–23.03) | 1.89 | 0.02202 | Tissue enriched (skin) |
| Annexin A8-like protein 1 | Q5VT79 | ANXA8L1 | 22.27 (21.4–25.36) | 20.01 (19.56–21.26) | 2.26 | 0.02793 | Group enriched (esophagus, skin, vagina) |
| Sodium/potassium-transporting ATPase subunit alpha-1 | P05023 | ATP1A1 | 22.94 (22.24–23.32) | 21.3 (21.09–22.28) | 1.64 | 0.03499 | Tissue enhanced (parathyroid gland) |
| Vinculin | A0A024QZN4 | VCL | 22.02 (21.77–22.82) | 23.63 (22.4–24.42) | −1.61 | 0.04347 | Low tissue specificity |
| Keratin 13 | A1A4E9 | KRT13 | 24.18 (23.3–24.74) | 27.66 (26.01–28.76) | −3.48 | 0.04347 | Tissue enhanced (esophagus, vagina) |