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. 2023 Jan 4;24(2):917. doi: 10.3390/ijms24020917

Figure 6.

Figure 6

Chemogenetic control of ADAM10 analog cleavage and activation. (A) Schematic drawing of Cyt-TEVcs ADAM10 before (veh) and after (rap) uniRapR-TEV activation. (B) Assessment of Cyt-TEVcs ADAM10 cleavage by WB analysis of lysates from HEK293T cells transfected with Cyt-TEVcs and uniRapR-TEV and treated with veh or rap. (C) uniRapR-TEV activation significantly increased Cyt-TEVcs shedding. (D) Schematic drawing of BS-Cyt-TEVcs before (veh) and after (rap) uniRapR-TEV activation. (E) Cleavage of BS-Cyt-TEVcs by uniRapR-secTEV assessed by WB. (F) In the presence of rap, a significant increase of BS-Cyt-TEVcs shedding was observed, indicating that uniRapR-TEV-mediated cleavage activated ADAM10 analog. Star indicates the sites of TEV-induced cleavage in the ADAM10 analogs. Data are expressed as mean ± SEM. ** p < 0.001; *** p < 0.0001; unpaired two-tailed Student’s t-test.