FIG. 3.

Aligned amino acid sequences representing the C-terminal segments of VSP H7 (H7) and subvariant VSPs derived from G. lamblia clone GS/M-83-H7. Sequences were deduced from cloned vsp cDNA derived from parasite populations recovered on days 0 (cDNA clones H7 and 0a), 7 (clones H7 and 7a to -c), 14 (clones 14a to -g), 21 (clones 21a to -f), and 42 (clones 42 a to -e) p.i. from infected mice or clones IVa to -h from negatively selected axenic trophozoites. Similarities between VSP H7 and the corresponding segments of the subvariant VSPs identified ranged between 83 (clone 7b) and 31% (clone 42d). Black, invariant positions; boxes, regions of high sequence similarities (conserved residues are in boldface); dots, alignment gaps; asterisks, positions with frequent occurrence of cysteines (C); bar, highly conserved transmembrane segment (positions 139 to 159 of VSP H7). GenBank accession numbers are at the right.