FIG. 4.

Phylogenetic relationship inferred from the C-terminal amino acid sequences of VSP H7, the subvariant VSPs identified in this study from cDNA, and corresponding VSP sequences available from GenBank. Segments corresponding to residues 29 to 159 (the C-terminal segment) of VSP H7 (Fig. 3) were aligned using ClustalX, version 1.8, and the resulting distance data were viewed using TreeView, version 1.6.1. Accession numbers of VSP sequences obtained for GS/M-83-H7-derived G. lamblia populations are given in Fig. 3. Those of the heterologous VSPs are as follows: AAD28789 (VSP417-4a/A-II), AAF31772 (VSP417-7/A-II), AAD04339 (VSP417-4b/A-II), AAF04387 (VSP417-7/A-I), AAG16629 (VSP9B10/A-I), A48579 (TSP11/A-I), AAD03497 (VSP417-3/A-II), AAD28790 (VSP417-2/A-II), AAD05040 (TSA417/A-II), A35502 (VSP9B10-LIKE), and AAF02907 (VSP417-6/A-I). Bar, index of dissimilarity (0.1 units) among the different sequences. Ellipses enclose sequences derived from the GS/M-83-H7 isolate (assemblage B) or from isolates belonging to genetic assemblage A and a third group of sequences apparently lying outside these assemblages.