Table 1.

Primer sequences used for quantitative real-time polymerase chain reaction.

Primer	Sequence
Mouse GAPDH	F: 5′-GTT GTC TCC TGC GAC TTC-3′
	R: 5′-GGT GGTCCA GGG TTT CTT-3′
Mouse COL1A1	F: 5′-CTG GAC AAC GTG GTG TGG-3′
	R: 5′-TGT TTG CCA GGT TCA CCA-3′
Mouse α-SMA	F: 5′-TCG GAT ACT TCA GCG TCA GGA-3′
	R: 5′-GTC CCA GAC ATC AGG GAG TAA-3′
Mouse MMP2	F: 5′-GCT GTA TTC CCG ACC GTT GA-3′
	R: 5′-TGG TCC GCG TAA AGT ATG GG-3′
Mouse TIMP1	F: 5′-ATC TCT GGC ATC TGG CAT CC-3′
	R: 5′-TTG CAG AAG GCT GTC TGT GG-3′
Mouse CCND1	F: 5′-CGC CCT CCG TAT CTT ACT TCA-3′
	R: 5′-TCT TCG CAC TTC TGC TCC TC-3′
Mouse Smo	F: 5′-GCG GGT TTG GCT TTT GAC-3′
	R: 5′-GGA CAC ATC CTG GGG CAA TA-3′
Mouse GLI1	F: 5′-GCC TTG AAA ACC TCA AGA CG-3′
	R: 5′-ATG GCT TCT CAT TGG AGT GG-3′
Mouse GLI2	F: 5′-AGT GGA ATG AGG TGA GTT CTG G-3′
	R: 5′-AGG CTG GCT TCT GTT GGA C-3′
Mouse HHIP1	F: 5′-GAA GGA GAT GCG AAG TTT GG-3′
	R: 5′-CCC TTC TCT TTA GGC GCT TT-3′
Mouse IHH	F: 5′-TCA AGG ACG AGG AGA ACA CG-3′
	R: 5′-ACC CGC AGT TTC ACA CCA-3′
Mouse SHH	F: 5′-CTG GTG ATC CTT GCT TCC TC-3′
	R: 5′-GGC TAA AGG GGT CAG CTT TT-3′
Mouse DHH	F: 5′-CAA GCA GTT TGT GCC CAG TA-3′
	R: 5′-GTC GGG GTT GTA GTT GGG TA-3′
Mouse PTCH1	F: 5′-GGA GCT CAG GCA ATA CGA AG-3′
	R: 5′-GGA GGC TGA TGT CTG GAG TC-3′
Mouse PTCH2	F: 5′-GCC TGC GTA ACA ATG CTG-3′
	R: 5′- GCA CAA AGC CCA AGA CCT -3′
Mouse VEGF	F: 5′-GTA CCT CCA CCA TGC CAA GT-3′
	R: 5′-TCA CAT CTG CAA GTA CGT TCG-3′
Mouse FGF2	F: 5′-AGC GGC TCT ACT GCA AGA AC-3′
	R: 5′-GCC GTC CAT CTT CCT TCA TA-3′
Mouse THBS1	F: 5′-CCA AAG CCT GCA AGA AAG AC-3′
	R: 5′-CCT GCT TGT TGC AAA CTT GA-3′

GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase; COL1A1, Collagen Type I Alpha 1 Chain; α-SMA, α-smooth muscle actin; MMP2, Matrix Metallopeptidase 2; TIMP1, Tissue inhibitor matrix metalloproteinase 1; CCND1, Cyclin D1; Smo, smoothened; GLI, GLI Family Zinc Finger; HHIP1, hedgehog-interacting protein 1; IHH, Indian Hedgehog Signaling Molecule; SHH, Sonic Hedgehog Signaling Molecule; DHH, Desert Hedgehog Signaling Molecule; PTCH, Patched; VEGF, Vascular endothelial growth factor; FGF2, Fibroblast Growth Factor 2; THBS1, Thrombospondin-1.