Figure 1.

C57BL/6J mice show less disease severity compared to BALB/c and Rag2^−/− mice following mouse-adapted SARS-CoV2 (MA10) infection. (A) Schematic overview of the experimental timeline for 10-week-old male C57BL/6, BALB/c, and Rag2^−/− mice. Animals were inoculated via the intranasal route with mock (PBS) or MA10 strain of SARS-CoV-2 (1 × 10⁵ TCID₅₀). Upon 3 days post-infection (3 dpi), mice were euthanized, and RNA isolated from the lung samples by the Trizol method was processed for gene expression. (B) Subgenomic-N viral copies and (C) Percent starting weight loss in SARS-CoV-2 (MA10)-infected C57BL/6 and SARS-CoV-2 (MA10) infected Rag2^−/− mice and BALB/c (D) Survival rate. Data were analyzed using 2-factor ANOVA followed by Sidak’s multiple comparisons if more than two groups. The Mann-Whitney U test is used to compare the difference in N viral copies between C57Bl/6J and Rag2^−/−. Error bars represent mean +/− SEM, * p < 0.05, n = 4–5/group.