Table 3.
Specification of the PCR primers and parameters applied in the present study.
| Virulence Factor Detected | Gene/PCR Primer Name |
Manufacturer | Primer Sequence 5′→3′ | Tm (°C) |
Annealing Temperature (°C) |
Amplicon Size (bp) |
|---|---|---|---|---|---|---|
| Exotoxin U | exoU F | Sigma | CCGTTGTGGTGCCGTTGAAG | 55.9 | 64 | 134 |
| exoU R | CCAGATGTTCACCGACTCGC | 55.9 | ||||
| Phospholipase C (non-hemolytic) | plC N F | Integrated DNA Technologies |
GTTATCGCAACCAGCCCTAC | 55.9 | 54 | 466 |
| plC N R | AGGTCGAACACCTGGAACAC | 57.2 | ||||
| Phospholipase C (hemolytic) | plC H F | GAAGCCATGGGCTACTTCAA | 55.1 | 50 | 307 | |
| plC H R | AGAGTGACGAGGAGCGGTAG | 58.2 | ||||
| Elastase B | lasB F | Genomed | GGAATGAACGAAGCGTTCTC | 51.8 | 50 | 300 |
| lasB R | GGTCCAGTAGTAGCGGTTGG | 55.9 | ||||
| Pilin A | pilA F | ACAGCATCCAACTGAGCG | 50.3 | 59 | 1675 | |
| pilA R | TTGACTTCCTCCAGGCTG | 50.3 | ||||
| Pilin B | pilB F | TCGAACTGATGATCGTGG | 48.0 | 56 | 408 | |
| pilB R | CTTTCGGAGTGAACATCG | 48.0 | ||||
| Neuraminidase 1 | nan1 F | AGGATGAATACTTATTTTGAT | 42.6 | 47 | 1316 | |
| nan1 R | TCACTAAATCCATCTCTGACCCGATA | 56.4 |