Figure 6.

Schematic diagram of multiplex PCR assay for detecting and genotyping P. insidiosum. The multiplex PCR requires several pairs of primers (represented as short blue, orange, and green lines) to simultaneously amplify multiple target sequences (i.e., different rDNA sequence regions of P. insidiosum) in one reaction tube. The number and sizes of the resulting amplicon can be assessed by gel electrophoresis, which aided in identifying and genotyping the organism in question. The diagram is adapted from the references [19,59].