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Evaluation of cellular uptake of LNPs. Cellular uptake of LNPs into Neuro2a cells was evaluated based on MFI using a FACS. The LNPs were labeled with DiD, a fluorescent dye, and the cells were analyzed after the LNP treatment. Data are represented as the mean ± S.D. (n = 3). ** p < 0.01, one-way ANOVA followed by Student–Newman–Keuls test.
