Figure 1.

SQV-loaded liposomes are effectively internalized by Mtb-infected human macrophages. (a) Flow cytometry analysis of rhodamine-labeled liposomes incorporated by macrophages infected with Mtb H37Rv after 3 h of treatment. The bars represent the mean percentage of macrophages positive for liposomes (left y-axis). The dots represent the median fluorescence intensity per macrophage for each donor (right y-axis) and the horizontal lines represent the mean of all donors. The histogram displays one representative experiment. Error bars depict the standard error of the mean calculated from 4 independent experiments. RFU—relative fluorescence units. (b) Fluorescence microscopy analysis of the same conditions analyzed in (a). Mtb H37Rv is depicted in green and SQV-loaded liposomes are depicted in red; the nucleus is contrasted in blue.