Figure 5.

Anticancer effects of Calystegia silvatica leaves and stems extracts in methanol on cancer cell lines: (A) complete monolayer sheets are seen in all of the cancer cell lines that have not been treated; (B) doxorubicin treatment results in rounded and shrunk cells in all of the cancer cell lines; (C) shrunk cells are observed in HeLa and MCF7 cell lines treated with the extract from Calystegia silvatica leaves; however, no morphological changes are observed in Caco2, HepG2 and WI38; (D) the extract from Calystegia silvatica stems used to treat HeLa, PC3, MCF7 and HepG2 cancer cell lines revealed significantly rounded and shrunk cells; however, small rounded and shrunk cells are observed in Caco2 and W138.