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Fixed aqueous layer thickness (in nm) obtained as the slope of the linear regression of ionic strength of the dispersing medium against NP surface charge: ln (ζ) against k (k = 3.3 c0.5, where k − 1 is the Debye length). There is a significant difference between M33_PEG2000 NPs and M33_PEG5000 NPs (one-way ANOVA; p ˂ 0.05), and between both the two PEGylated formulations and the PLGA NP formulation control (one-way ANOVA; p ˂ 0.0001).
