In vitro aerosol performance of M33_PEG 2000-Rhod and M33_PEG 5000-Rhod delivered through Aeroneb® Go and PARI TurboBOY nebulizers: Cumulative mass recovered was a function of the cut-off diameter of the NGI stages (A,B), NGI deposition pattern (A’,B’). The emitted dose was measured as the difference between the total amount of NPs initially placed in the nebulizer chamber and the amount remaining. At the flow rate used (15 L/min), the cut-off diameters of the NGI stages were 0.98 µm (Cup 1), 1.36 µm (Cup 2), 2.08 µm (Cup 3), 3.3 µm (Cup 4), 5.39 µm (Cup 5), 8.61 µm (Cup 6), 14.1 µm (Cup 7). No significant differences were observed for all the formulations tested (one-way ANOVA multiple comparison; p > 0.05).