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. 2022 Dec 20;11(1):2. doi: 10.3390/vaccines11010002

Figure 3.

Figure 3

Biological activity of FL PCSK9-SpyT antigen by LDLR staining on Hepa1-6 cells. Hepa1-6 cells incubated with growth media (control), 100 nM FL muPCSK9 or 100 nM FL muPCSK9-SpyT for 4 h at 37 °C. Cells were stained with DAPI (blue) and anti-LDLR-FITC (green). (A). Representative cells are shown as merged pictures (top) or FITC signal alone (bottom). The size bar is 50 µm. (B). The relative fluorescence unit (RFU) was determined (n = 65) by Cytation5 software and depicted as mean ± SD. Statistical analysis was performed on log-transformed RFU values using one-way ANOVA, Tukey’s multiple comparisons test (adjusted p-value < 0.05 was accepted as significant, ns > 0.05, **** < 0.0001).