Table 1.
Sequences of the primers used for target genes.
| Screened Gene | Primer | Primer Sequences (5′------->3′) | Expected Sizes (bp) |
|---|---|---|---|
| cnf1 | Cnf1 | 5′-aagatggagtttcctatgcaggag-3′ | 498 |
| Cnf2 | 5′-cattcagagtcctgccctcattatt-3′ | ||
| sat | SAT F | 5′-ggtattgatatctccggtgaac-3′ | 779 |
| SAT R | 5′-atagccgcctgacatcagtaat-3′ | ||
| papG II/III | pF f | 5′-ctgtaattacggaagtgatttctg-3′ | 1070 |
| pG r | 5′-actatccggctccggataaaccat-3 | ||
| iucD | iucD f | 5′-aaaactgacatcggatggc-3′ | 253 |
| iucD r | 5′-gtatttgtggcaacgcagaa-3′ | ||
| fyuA | FyuA f’ | 5′-tgattaaccccgcgacgggaa-3′ | 880 |
| FyuA r’ | 5′-cgcagtaggcacgatgttgta-3 | ||
| focG | FocG f | 5′-cagcacaggcagtggatacga-3′ | 360 |
| FocG r | 5′-gaatgtcgcctgcccattgct-3′ | ||
| sfaS | SfaS f | 5′-gtggatacgacgattactgtg-3′ | 240 |
| SfaS r | 5′-ccgccagcattccctgtattc-3′ | ||
| iroN | IRON1 | 5′-tattcgtggtatggggccgga-3′ | 547 |
| IRON2 | 5′-gcccgcatagatattcccctg-3′ | ||
| hlyA | Hly f | 5′-aacaasgataagcactgttctggct-3′ | 1177 |
| Hly r | 5′-accatataagcggtcattcccrtca-3′ |
Cnf1: cytotoxic necrotizing factor type 1, SAT: secreted autotransporter toxin, Sfas: S-fimbriae adhesin, Pap G: adhesin P-fimbria, Hly: hemolysin RTX, Foc G: F1C fimbrial adhesin, Fyu A: yersiniabactin outer membrane receptor, IUCD: aerobactin biosynthesis receptor, IronB: salmochelin outer membrane receptor.