Figure 2.

Mice vaccinated with Mt10 vaccine virus show enhanced protection against diabetes incidence. (a) Blood glucose. Blood glucose levels were monitored weekly, and diabetes incidence is shown. Mice with a blood glucose level of >300 mg/dL on two consecutive readings were deemed diabetic. Data sets obtained from each group involving n = 5–9 mice are shown. Log-rank test with Bonferroni correction was used to compare diabetes incidence. (b) Viral mRNA. Groups of mice were given saline or were vaccinated with Mt10 virus, and 7 days later were challenged with or without CVB4. Pancreata were collected 5 weeks post-challenge, total RNA was extracted, and viral RNA was estimated by RT-qPCR using VP1-specific nucleotide sequences. After normalizing the expression levels of viral RNA relative to GAPDH, 2^{−(∆∆Ct)} values were calculated. Mean ± SEM values representing 5–9 samples per group are shown. Mann–Whitney U test and one-way ANOVA with Tukey’s multiple comparison test was used to determine significance between groups. **** p ≤ 0.0001.