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. 2023 Jan 13;28(2):818. doi: 10.3390/molecules28020818

Figure 1.

Figure 1

Knockdown of STIM1 inhibits the EGF-stimulated migration of 5–8F cells. (A) The expression levels of STIM1 in 5–8F cells transduced with shRNA-Ctrl (nonsense) or shRNA-STIM1 vector were determined by RT-qPCR. (B) The expression levels of STIM1 in the shRNA-Ctrl or shRNA-STIM1 5–8F cells were detected by Western blotting. (C) The intracellular STIM1 aggregation was analyzed by immunofluorescence staining upon EGF stimulation. Bar = 10 μm. (D) Wound-healing assay was performed to evaluate the effect of STIM1 knockdown on EGF-stimulated cell migration. Representative photographs of migratory cells were captured after incubation of cells with extracellular EGF for 24 h. Bar = 100 μm. Cell migration was quantified by calculating the cell-covered area (right) (n = 5 per group). (E) The transwell assay was performed to evaluate cell migration. Representative photographs of the membrane-penetrating cells stained with Giemsa from each group are shown. The average numbers of membrane-penetrating cells from a random field are shown (right panel) (n = 6 per group). Data are presented as mean ± SD of three independent experiments. (* p < 0.05, Student’s t-test).