Figure 5.

Afatinib combined IR treatment inhibited the EMT process. (A,B) 5-8F (A) and HNE2 cells (B) were treated as indicated and cell migration was measured by wound healing assay. (C) 5-8F and HNE2 cells were treated as indicated and cell migration was assessed by transwell assay without Matrigel. Cellular migration was plotted as the average number of cells in three different areas. (D) 5-8F and HNE2 cells were treated as indicated, WCLs were prepared at 24 h post-treatment and analyzed by immunoblotting with representative EMT marker antibodies. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001. Error bars represent ±SD of triplicate experiments.