Fig. 1.

Chromatographic and GC analysis of HAEGS. A Chromatographic separations of Gymnema Sylvestre plant extract was carried out on a Surveyor UHPLC system using a Syncronis C18 column (2.1 × 100 mm) with a gradient mobile phase of acetonitrile and 0.1% formic acid in water with a flow rate of 0.15 mL/min. In the LC–HRMS analysis, a total of 20 plant secondary metabolites were identified based on the derived elemental compositions from the high-resolution mass spectral data. Thermo Xcalibur software (version 2.2) was used for processing the mass spectral data. B The GC analysis was performed with Agilent 6890 GC equipped with Agilent Technologies5977A mass selective detector (MSD). The separation of analytes was carried out on capillary column (Agilent) HP-5% Phenyl Methyl Siloxane (30 m × 250 um × 0.5 um). The volatile organic compounds were identified by using the W9N11.L, NIST14 library database. In this analysis, a total of 19 plant secondary metabolites were identified. Mass spectral data were processed using MSD ChemStation