Fig. 2.

HAEGS treatment mitigated the LPS-induced pro-inflammatory and chemokine marker’s expression in RAW 264.7 and epithelial cells. A–G RAW-264.7 cells were cultured in serum-free media for 6 h, then cells were pre-treated with HAEGS (125 and 250 μg/mL) or dexamethasone (100 ng/mL and 500 ng/mL), for a period of 2 h; then cells were stimulated with LPS (1 µg/mL) for a period of 12 h. H–K BEAS-2B cells were pre-treated with HAEGS (125 and 250 μg/mL) or DEXA (100 ng/mL and 500 ng/mL) for 2 h; further, cells were stimulated with LPS (5 µg/mL) for another 12 h. Thereafter, cells were subjected to qRT-PCR analysis using specified primer sets. Graphs in panels represent fold change in gene expression normalized to β2M. *p < 0.05, **p < 0.01, ***p < 0.001. NS non-significant. One-way ANOVA was performed for statistical analysis. Data represented as mean ± SEM, n = 3