Fig. 3.

HAEGS treatment mitigated the oxidative stress by modulating the MAPK and NF-κB pathways. A, B RAW-264.7 (A) or BEAS-2B (B) cells were pre-treated with HAEGS (125 and 250 μg/mL) or DEXA (100 ng/mL and 500 ng/mL) for 2 h, then cells were stimulated with LPS (1 µg/mL or 5 µg/mL) or for another 12 h. Thereafter, cells were trypsinized and subjected to DCFDA analysis using flow cytometry. C–G RAW-264.7 (C–E) or BEAS-2B (F–G) cells were pre-treated with HAEGS (125 and 250 μg/mL) and DEXA (100 ng/mL and 500 ng/mL) after 2 h, and cells were stimulated with LPS (1 µg/mL) for another 12 h. Further, cells were subjected to Western blot analysis using specified antibodies. Protein expressions were quantified using Image J software and graphs were plotted against each specified protein marker