Fig. 7.

HAEGS treatment ameliorated the infiltration of inflammatory cells, pathological changes and neutrophil-mediated events in LPS-stimulated lung tissues. After the treatment period, the lungs were subjected to histopathology. A Representative images (10 ×) of H&E staining, the red arrow points at typical areas of inflammation, manifesting oedema and infiltration of inflammatory cells. Graphs represent the score of (E) lung injury and inflammation score. PAS staining was used to observe goblet cells in LPS-induced rats with acute lung injury. B Representative images (10x) of PAS staining. Goblet cells were identified using PAS in LPS-induced rat lungs. F The quantification of the PAS-positive score. C, D, G and H Representative IHC images of neutrophil elastase (C) and myeloperoxidase (D), quantification of neutrophil elastase expression (G) and quantification of MPO expression (H) in the lung sections. Images were taken under 20 × magnification. n = 8 *p < 0.05; **p < 0.01; ***p < 0.001 vs. the LPS control group. Data represented as mean ± SEM. One-way ANOVA was performed for statistical analysis