Fig. 8.

Fraction 6 metabolites significantly inhibited the IL-6 and IL-1β expression levels in BEAS-2B cells. A and B BEAS-2B cells were cultured in serum-free media for 6 h, then cells were pre-treated with various fractions of HAEGS (25 μg/mL) or deacyl gymnemic acid or gymnemagenin or (100 μg/mL) or whole HAECS (125 μg/mL) for a period of 2 h, then cells were stimulated with LPS (5 µg/mL) for a period of 12 h. Thereafter, cells were subjected to qRT-PCR analysis using specified primer sets. Graphs in panels represent fold change in gene expression normalized to β2M. C) Molecular composition of fraction 6 was carried out by loading the fraction 6 of HAEGS chromatographic separations on a Surveyor UHPLC system using a Syncronis C18 column (2.1 × 100 mm; Thermo Scientific, Germany) column with a gradient mobile phase of 0.1% formic acid in water and acetonitrile with a flow rate of 0.15 mL/min. *p < 0.05, **p < 0.01, ***p < 0.001. NS non-significant. One-way ANOVA was performed for statistical analysis. Data represented as mean ± SEM, n = 3