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Representative RNSacope images, illustrating that low-density CB1 mRNA is colocalized with GAD1, a GABAergic neuronal marker, in the medial VTA, suggesting CB1R expression in GABA neurons. A, CB1 mRNA-staining; B, GAD1 mRNA-staining; C, CB1 and GAD1 colocalization in DAPI-labeled cells.
