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Representative triple-labeled images, illustrating CB1-TH-VgluT2 colocalization in most of TH+ DA neurons in the medial VTA. A, TH mRNA-staining; B, CB1 mRNA-staining; C, VgluT2 mRNA-staining; D, CB1-TH-VgluT2 co-localization in DAPI-labeled cells. Notably, CB1 mRNA was also detected in VgluT2+ only glutamate neurons.
