Table A2.
Longitudinal study graphs showing the effect of hospital treatment on FCM (faecal cortisol metabolite) values measured by 50c (continuous line) and cortisol (dashed line) EIAs.
Graphs Reflecting FCM Values | Notes and Correlations |
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Locket (female): control The relatively high values on day 1 are likely related to the hospitalisation the afternoon before day 1. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = −0.0385 (p = 0.9347) |
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Scratchy (male; orphan): control The high values on day 1 are likely related to the hospitalisation the day before day 1 after she was found alone on the side of a road. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = −0.18442 (p = 0.5818) |
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Cloyna (male): diseased (cystitis, anaemia and kidney disease) He had extreme values of FCMs at admission due to illness. The spike on day 2 is likely related to the hospitalisation on day 1. He was euthanised after the last faeces collection. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = −0.0019 (p = 0.9962) |
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Cyril (female): diseased (Chlamydia; LAMP = 13.37 min) The spike on day 2 is likely related to the hospitalisation on day 1. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.1000 (p = 0.8311) |
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Larissa (female): diseased (Chlamydia; LAMP = 3.56 min) The high values from day 7 are likely related to the start of the injection treatment. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = −0.6866 (p = 0.0600) |
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Molly (female): diseased (Chlamydia; LAMP = 15.15 min) Treatment started on day 4. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.3144 (p = 0.6064) |
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Golf (male): diseased (Chlamydia; LAMP = 14.4 min) The spikes on days 2 and 6 are likely related to the procedure for general anaesthetic carried out on days 1 and 5. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.4136 (p = 0.2348) |
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Clementine (female): diseased (Chlamydia: LAMP = 8.51 min) No specific data were recorded but she was ultimately euthanised. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.8439 (p = 0.0084) |
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Rarsta (male): diseased (kidney infection) He underwent injection treatment. Rarsta was euthanised 3 days after the last scat collection. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r= 0.4579 (p = 0.2539) |
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Blake (male): diseased (chlamydial conjunctivitis) The spike on day 4 is likely related to day 3 injection. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r= 0.8270 (p = 0.0113) |
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Spring (female): diseased (chlamydial conjunctivitis) She was recorded to be highly stressed in her enclosure on day 3. No notes were available to explain the increase on day 7. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.4835 (p = 0.2249) |
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Wayne-o (male): injured (hit by car) The spike on day 2 is likely related to the hospitalisation on day 1. Procedure for general anaesthetic was carried out on day 5. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = −0.1518 (p = 0.7197) |
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Firecracker (female): injured (hit by a car) The spike on day 2 is likely related to the procedure for general anaesthetic carried out on day 1. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.4041 (p = 0.2808) |
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Milko (male): injured (hit by a car) Injection treatment started on day 1. Procedure for general anaesthetic was carried out on days 2 and 4. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.1905 (p = 0.6235) |
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Farrel (male): injured (abscess on the face and peritonitis) He was administered an injection of antibiotic on day one and carprofen, starting on day 2 for 5 days. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.2169 (p = 0.6403) |
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Luca (male): injured The high values on day 1 are likely related to the hospitalisation in the afternoon before day 1. No data were recorded that may explain the spike on day 4. The higher values from day 7 are likely related to the procedure for general anaesthetic carried out on day 7. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.3763 (p = 0.3582) |
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Noah (male): injured (dog attack) The spike on day 2 is likely related to the hospitalisation in the afternoon before day 1. Noah was kept in the ICU. No other information is available that could explain the spike on day 7. Correlation coefficient between FCM values measured by the cortisol and 50c EIA: Pearson’s r = 0.6886 (p = 0.0699) |