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. 2001 Sep;69(9):5385–5394. doi: 10.1128/IAI.69.9.5385-5394.2001

FIG. 6.

FIG. 6

Transcriptional analysis of prpL. (A) Promoter region of the prpL gene. The Shine-Dalgarno (S/D) site, the start site of the coding sequence, and the putative transcriptional start sites of T1 and T2 are indicated. The 422- and 727-nt probes used for RNase protection analysis of the prpL gene are shown by black bars. The consensus sequence for the proposed iron starvation box is indicated. (B) RNase protection analysis of P. aeruginosa PAO1 and ΔpvdS::Gm RNAs isolated at the time points shown from cells grown aerobically and microaerobically (5% oxygen) under low (−)- or high (+)-iron conditions and probed with a 422-base riboprobe. The positions of 100- and 200-base RNA size standards are indicated. (C) RNase protection analysis of P. aeruginosa PAO1, ΔpvdS::Gm, and ΔptxR::Gm. RNA was isolated at 10 h from cells grown aerobically under low (−)- and high (+)-iron conditions and probed with a 727-base riboprobe. The relative intensities of the transcripts in panels B and C were quantified with a Bio-Rad Personal FX phosphorimager using Quantity One software (version 4.0.3) from Bio-Rad. The sizes of the RNA fragments in the marker (M) lane are shown.