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Absorption (a) and circular dichroism (b) spectra of conventional liposomal AmB (LAmB) prepared according to [77], before and after 4 h heating at 60 °C, in comparison to AmBisome®. Lyophilized LAmB was reconstituted with water at 4 mg/mL AmB and submitted or not to heat curing. Spectra were registered at 25 °C after reconstitution of the nanoformulations in water and 100-fold dilution in PBS.
