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. 2022 Dec 28;15(1):84. doi: 10.3390/v15010084

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LAMP primer design. Schematic showing location of primer recognition sites around the N4R gene, which is duplicated in left and right inverted terminal repetitions (ITR) of the mpox genome. The positions from 5840nt/190765nt to 6339nt/191264nt are based on the complete genome of MPVX-FRA-2022-TLS67 (GenBank accession: ON602722.2). Outer primers (F3, B3) are indicated by red line arrows, internal primers (BIP and FIP) are indicated by green and purple line arrows, loop primers (LB, FB) are indicated by blue line arrows.