Effect of the addition of antivenom (AV; 20 µL) and Varespladib (0.8 µM), 60 min post venom, on the myotoxic effects of venom (10 µg/mL). Arrows indicate the time of addition of combined treatment. (a) Effect on direct twitches; * p < 0.05, significantly different to Varespladib alone; one-way ANOVA followed by Bonferroni’s post hoc test. (b) Effect on baseline tension; * p < 0.05, significantly different from vehicle response; # p < 0.05, significantly different from venom alone; all one-way ANOVA followed by Bonferroni’s post hoc test. n = 4–6 where n is the number of preparations from different animals; error bars indicate standard error of the mean.