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IFN-γ (A) and IL-2 (B) production in LN cell and spleen cell cultures from unvaccinated and vaccinated B6 mice infected with 3 × 104 M. ulcerans strain 5150 AFB. Cells were restimulated for 3 days in vitro with bovine PPD (grey bars), BCG CF (white bars), or purified Ag85 (black bars). Data are expressed as mean numbers of picograms per milliliter ± SD (IFN-γ) and as mean counts per minute ± SD (IL-2) in culture supernatants (tested in duplicate) from pools of five mice.
