Figure 2.

Biochemical comparison of different groups in rat skin investigation. (A) Collagenase; (B) elastase; (C) hyaluronidase; (D) tyrosinase; (E) nitric oxide (NO); (F) malondialdehyde (MDA); and (G) cyclooxygenase2 (COX-2) levels in five rat groups. Rats in the control group (C) were untreated; rats in the skin inflammation group were injected with an intramuscular dose of phenobarbital at 50 mg/kg body mass (bm) once per week for three weeks, plus a daily dose of intracutaneous formaldehyde at 6 µL/kg body mass (bm); rats in the PSOE group received a daily oral dose of PSOE at 250 mL/kg bm for 21 days; rats in the skin inflammation + PSOE group were injected with phenobarbital, formaldehyde, and PSOE at the same dosages and in the same manner as the phenobarbital, formaldehyde, and PSOE groups for three weeks; additionally, 250 mg/kg PSOE treatment was administered directly to the inflamed skin once per day for 21 days of treatment; rats in the skin inflammation + Avocom-M group were injected with phenobarbital and formaldehyde at the same dosages and in the same manner as the phenobarbital and formaldehyde groups for three weeks; we also administered Avocom-M 250 mg/kg treatment directly to the inflamed skin once per day for 21 days of treatment. Data are given as mean ± SD for 8 rats. Statistical significance was set to p ≤ 0.05; means reported with the same letters (a, b, c, d) were not significantly different.