TABLE 3.
Antigen-specific proliferation of T cells from VW7-3-immunized mice
| Stimulationa | Stimulation indexb for:
|
|
|---|---|---|
| Naive mice | Immune mice | |
| RPMIc | 1 | 1 |
| RPMI + VW7-3 | 7.44d | 15.7d |
| RPMI + VW2-1 | 3.5d | 7.1d |
| RPMI + VW3-3 | 3.22d | 4.6d |
| RPMI + VW4-1 | 1.11 | 1.2 |
a
Proliferation was assayed with T cells from immunized and naive mice stimulated with the recombinant fragments of Pmy as described in Materials and Methods.
b
Reported values are stimulation indexes calculated from the means of radioactivity incorporation (quadruplicates) for each experimental group after an 18-h pulse with [3H]thymidine.
c
Absolute values were 1.8 ± 0.3 and 1.5 ± 0.3 (kcpm ± standard deviation) for naive and immune medium controls, respectively.
d
Absolute values for each experimental group were statistically different from absolute values for RPMI-stimulated control cells (P < 0.05).