Table 2.

The minimum incubation time (h) necessary to get accurate estimates for $D_{\max }$ and $D{C}_{50}$ (i.e., estimates that are less than a factor of 1.5 ($D{C}_{50}$) or less than 5% ($D_{\max }$) different from the steady-state values) is given for different threshold values ($D_{\max }\ge x$) and different proteins ($t_{\text{½},\mathrm{P}}\le x$). A green box indicates that 24 h should be sufficient, while orange means that it takes longer than that. The required incubation time decreases with increasing $D_{\max }$ and decreasing $t_{\text{½},\mathrm{P}}$.

	${\mathit{D}}_{\max } \left(\mathbf{\%}\right)$
${\mathit{t}}_{\mathrm{½}, \mathsf{P}} \left(\mathsf{h}\right)$	30	50	60	70	75	80	85	90	95	99
2	5	4	4	3	3	3	3	3	3	3
4	9	8	7	6	6	6	6	5	5	5
6	13	11	10	9	9	9	8	8	7	7
12	26	22	20	18	18	17	16	15	14	13
24	51	44	40	36	35	33	31	29	27	26
36	77	66	60	54	52	49	46	43	41	38
48	102	87	80	72	69	65	61	58	54	51
72	153	131	120	108	103	97	92	86	81	76
96	204	174	159	144	137	130	122	115	107	101
192	407	348	318	288	273	259	244	229	214	202